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alexa fluor 647 (a647) fluorescent dye  (Thermo Fisher)


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    Thermo Fisher alexa fluor 647 (a647) fluorescent dye
    CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL <t>A647-labeled</t> collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.
    Alexa Fluor 647 (A647) Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alexa+fluor+647+(a647)+fluorescent+dye/pmc08841889-213-3-9
    Average 90 stars, based on 1 article reviews
    alexa fluor 647 (a647) fluorescent dye - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Uncovering mediators of collagen degradation in the tumor microenvironment"

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment

    Journal: Matrix Biology Plus

    doi: 10.1016/j.mbplus.2022.100101

    CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL A647-labeled collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.
    Figure Legend Snippet: CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL A647-labeled collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.

    Techniques Used: Cell Culture, Labeling, Flow Cytometry, Fluorescence

    Related Articles

    Incubation:

    Article Title: Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs.
    Article Snippet: CD1d-restricted natural killer T (NKT) cells are a unique lymphocyte population that makes important contributions to host defense against numerous microbial pathogens.. The powerful immunomodulatory effects of these cells can be exploited in mice by cognate antigens for multiple therapeutic purposes, including for protection from infectious diseases and as adjuvants to improve vaccines against microbial organisms.. These applications have potential to treat and prevent infectious diseases in livestock species that express NKT cells, including pigs.

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment
    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

    Cell Culture:

    Article Title: Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs.
    Article Snippet: CD1d-restricted natural killer T (NKT) cells are a unique lymphocyte population that makes important contributions to host defense against numerous microbial pathogens.. The powerful immunomodulatory effects of these cells can be exploited in mice by cognate antigens for multiple therapeutic purposes, including for protection from infectious diseases and as adjuvants to improve vaccines against microbial organisms.. These applications have potential to treat and prevent infectious diseases in livestock species that express NKT cells, including pigs.

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment
    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

    Labeling:

    Article Title: Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs.
    Article Snippet: CD1d-restricted natural killer T (NKT) cells are a unique lymphocyte population that makes important contributions to host defense against numerous microbial pathogens.. The powerful immunomodulatory effects of these cells can be exploited in mice by cognate antigens for multiple therapeutic purposes, including for protection from infectious diseases and as adjuvants to improve vaccines against microbial organisms.. These applications have potential to treat and prevent infectious diseases in livestock species that express NKT cells, including pigs.

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment
    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

    Flow Cytometry:

    Article Title: Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs.
    Article Snippet: CD1d-restricted natural killer T (NKT) cells are a unique lymphocyte population that makes important contributions to host defense against numerous microbial pathogens.. The powerful immunomodulatory effects of these cells can be exploited in mice by cognate antigens for multiple therapeutic purposes, including for protection from infectious diseases and as adjuvants to improve vaccines against microbial organisms.. These applications have potential to treat and prevent infectious diseases in livestock species that express NKT cells, including pigs.

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment
    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

    Fluorescence:

    Article Title: Adjuvant effects of therapeutic glycolipids administered to a cohort of NKT cell-diverse pigs.
    Article Snippet: CD1d-restricted natural killer T (NKT) cells are a unique lymphocyte population that makes important contributions to host defense against numerous microbial pathogens.. The powerful immunomodulatory effects of these cells can be exploited in mice by cognate antigens for multiple therapeutic purposes, including for protection from infectious diseases and as adjuvants to improve vaccines against microbial organisms.. These applications have potential to treat and prevent infectious diseases in livestock species that express NKT cells, including pigs.

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment
    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.



    Similar Products

    alexa fluor 647 (a647) fluorescent dye  (Thermo Fisher)
    90
    Thermo Fisher alexa fluor 647 (a647) fluorescent dye
    CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL <t>A647-labeled</t> collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.
    Alexa Fluor 647 (A647) Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alexa+fluor+647+(a647)+fluorescent+dye/pmc08841889-213-3-9
    Average 90 stars, based on 1 article reviews
    alexa fluor 647 (a647) fluorescent dye - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL A647-labeled collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.

    Journal: Matrix Biology Plus

    Article Title: Uncovering mediators of collagen degradation in the tumor microenvironment

    doi: 10.1016/j.mbplus.2022.100101

    Figure Lengend Snippet: CAFs and MR + TAMs are the main cell types in collagen internalization. (A-C) Quantification of collagen internalization by cell types of the tumor microenvironment: Single-cell suspensions of LL/2 tumors (A), LL/2-GFP tumors (B) or EO771.LMB-GFP tumors (C) were cultured overnight with soluble 10 µg/mL A647-labeled collagen type I. Flow cytometry analysis was then performed to determine the internalized fluorescence in CAFs, TAMs, dendritic cells, granulocytes, monocytes, cancer cells, CD45 − CD31 − FAP − cells and CD45 − CD31 − FAP − GFP − cells. n = 2–6. Error bars = SEM. (D) Representative density plots from flow cytometry analysis of LL/2 tumor showing how TAMs were divided into subpopulations negative and positive for MR. Gates were based on isotype control. (E) Representative overlay of histograms from flowcytometry analysis of LL/2 tumors showing the level of internalized collagen in TAMs negative and positive for MR. (F-G) Quantification of collagen internalization by MR + macrophages, MR − macrophages and FAP + fibroblasts from LL/2 (F) and EO771.LMB tumors (G). n = 3–5, except CAFs in 3F ( n = 2). Error bars = SEM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way ANOVA with post hoc Tukey’s multiple comparisons test.

    Article Snippet: A vial of Alexa Fluor 647 (A647) fluorescent dye (Life Technologies) dissolved in 0.5 mL PBS was immediately added and incubated with gentle shaking for two hours at room temperature.

    Techniques: Cell Culture, Labeling, Flow Cytometry, Fluorescence